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Because free cysteine rarely occurs on protein surface, it is an excellent choice for chemoselective modification. Under basic condition, the cysteine residues will be deprotonated to generate a thiolate nucleophile, which will react with soft electrophiles, such as maleimides and iodoacetamides (shown in the first two reactions in Figure 2 below). Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days. Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Abstract: The reaction of ninhydrin with primary amino groups to form the purple dye now called Ruhemann's purple (RP) was discovered by Siegfried Ruhemann in 1910. In addition, imines such as pipecolic acid and proline, the guanidino group of arginine, the amide Free unique 2shared photo collection on any topic are waiting for you. Portraits, landscapes and funny pictures, random shots and entire works of photo art are all available just now. Browse our photo gallery and be sure to find photos interesting just for you. Besides, with 2shared, it is so easy to share your photos with other people.
In chemical synthesis, "click" chemistry is a class of biocompatible small molecule reactions commonly used in bioconjugation, allowing the joining of substrates of choice with specific biomolecules.
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22 Jan 2010 Download PDF Such bioconjugation techniques usually rely on selective coupling reactions to the side chains of specific amino acid
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Semiconductor quantum dot, peptide, DNA, nanocrystal, bioconjugation, iodoacetyl, sulfhydryl, washed as before to yield the free terminal primary amine.
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